Cost-effective cryopreservation without compromise
A new blog has been published by Amsbio comparing the benefits of its CELLBANKER® range of cryopreservation solutions with ‘homebrew’ cryopreservation media.
Homebrew cryopreservation media are do-it-yourself solutions, commonly using a mixture of DMSO, a serum (such as FBS), and a basal growth medium. These mixtures are often favored because of their lower upfront cost or to meet specific lab needs, but the optimal formulation depends heavily on the cell line’s sensitivity.
From academic research to GMP manufacturing, reproducibility, and reliability in cryopreservation are critical. While homebrew cryopreservation mixtures can work, variability between serum lots and preparation methods can lead to inconsistent results. Each batch needs revalidation, and contamination risks increase with manual handling. By comparison, CELLBANKER® eliminates that uncertainty. Each batch is manufactured under strict quality control using chemically defined, sterile components to deliver the same performance every time. It is also completely ready-to-use, removing the need for in-lab mixing and reducing contamination risk.
In addition, while CELLBANKER® provides an animal-free, chemically defined, sterile, and traceable solution that supports regulatory compliance and long-term sample integrity – homebrew serum-based media can introduce biological variability. The authors show how for sensitive cells such as primary cells, iPSCs, or organoids, the level of consistency provided by CELLBANKER® results in better cell viability and reproducibility post-thaw.
Beneficially the CELLBANKER® range also includes a GMP-grade formulation available at a competitive price point, allowing researchers to use clinical-grade materials early in development without a steep cost jump. This seamless transition from research to clinical manufacturing helps maintain consistency, simplifies validation, and avoids the expensive requalification often required when switching suppliers later in the process.
This informative blog also includes customer technical paper references and CELLBANKER® testimonials from internationally renowned organoid and stem cell research and development groups. To read the blog in full please visit https://www.amsbio.com/news/cellbanker. For further information on the CELLBANKER® range of cryopreservation solutions please visit https://www.amsbio.com/cryopreservation/ or contact Amsbio on +31-72-8080244 / +44-1235-828200 / +1-617-945-5033 / info@amsbio.com.
Now part of the Europa Biosite group of companies, AMS Biotechnology (Amsbio) is recognized as a leading transatlantic company contributing to the acceleration of discovery through the provision of cutting-edge life science technology, products, and services for R&D in the medical, nutrition, cosmetics, and energy industries. Amsbio has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion, and proliferation. This expertise in cell culture and the ECM allows Amsbio to partner with clients in tailoring cell systems to enhance organoid and spheroid screening outcomes using a variety of 3D culture systems, including organ-on-a-chip microfluidics. For drug discovery research, Amsbio offers assays, recombinant proteins, and cell lines. Drawing upon a huge and comprehensive biorepository, Amsbio is widely recognized as a leading provider of high-quality tissue specimens (including custom procurement) from both human and animal tissues. The company provides unique clinical grade products for stem cells and cell therapy applications. This includes GMP cryopreservation technology, and high-quality solutions for viral delivery.
Image caption: Murine Thyroid Organoids frozen in CELLBANKER® 1 during the lockdown and imaged post-thaw. IF staining for DAPI, Pax8 (marker of thyroid gland organogenesis), and Phalloidin (white/magenta/green). Image courtesy of the Clevers Lab, Hubrecht Institute.
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